Membrane Protein Complexes in E. coli
List of all membrane protein complexes in Escherichia coli, strain K12, that are listed on Complex Portal. For complexes where the stoichiometry of one or more protein was unknown, various different cases were modelled and what was considered most plausible is displayed here. In these cases, the stoichiometry is listed on the card. Note that some predictions do not align with the stoichiometry given on Complex Portal.
Click the accession code (CPX-0000) to view the Complex Portal entry. Clicking the image of the complex opens the Mol* interactive viewer in a new tab. Within this, the structure with and without the signal peptide can be viewed (if applicable), as well as the structure placed within a membrane; these can be toggled in the ‘State Tree’ on the left-hand-side menu.
AlphaFold confidence scores, ipTM and pTM, are displayed; AlphaFold pLDDT colouring can be seen in Mol*. The Download button fetches the complex together with its MemPrO and CG2AT membranes.
Inner Membrane Complexes
CPX-3569
CcmFH system I cytochrome c biogenesis complex
With 1 copy each of ccmF and ccmH
ipTM 0.74 pTM 0.82
CPX-4265
AcrEF-TolC multidrug efflux transport complex
With 6 copies of acrE and 3 copies of acrF
ipTM 0.75* pTM 0.77*
CPX-5782
Tol-Pal cell envelope complex (inner membrane component)
With 5 copies of tolQ, 2 copies of tolR, and 2 copies of the transmembrane domain of tolA
ipTM 0.7 pTM 0.73
CPX-5890
Translocation and assembly module complex
With 1 copy each of tamA and tamB
ipTM 0.59 pTM 0.49
CPX-5942
Galactitol-specific enzyme II complex
With 2 copies each of gatA, gatB, and gatC
ipTM 0.42 pTM 0.52
CPX-5968
D-mannose-specific enzyme II complex
With 3 copies each of manX, manY, and manZ
ipTM 0.64 pTM 0.67
CPX-5990
sgcABC sugar permease enzyme II complex
With 2 copies each of sgcA, sgcB, and sgcC
ipTM 0.43 pTM 0.55
CPX-6021
SdsRQP multidrug transport complex
With 3 copies of mdtP, and 2 copies each of mdtN and mdtO
ipTM 0.41 pTM 0.48
CPX-4681
YiaMNO tripartite ATP-independent periplasmic transporter complex
With 1 copy each of yiaM, yiaN, and yiaO
ipTM 0.79 pTM 0.8
CPX-5892
PspBC inner membrane stress response complex
With 2 copies each of pspB and pspC
ipTM 0.28 pTM 0.33
CPX-5969
Glucitol/sorbitol enzyme II complex
With 3 copies each of srlA, srlB, and srlE
ipTM 0.5 pTM 0.51
CPX-5994
Frw fructose-like enzyme II complex
With 4 copies each of ptsA and frwB, and 2 copies each of frwC and frwD
ipTM 0.5 pTM 0.54
CPX-2242
mlc-EIIB transcriptional regulator complex
With 2 copies of mlc and 2 copies of ptsG
ipTM 0.48 pTM 0.54
CPX-3445
Twin-arginine translocation complex
With 3 copies each of tatA, tatB, and tatC
ipTM 0.51 pTM 0.57
CPX-3568
CcmABCDE system I cytochrome c biogenesis complex
With 2 copies each of ccmB, ccmC, ccmD, ccmE
ipTM 0.81 pTM 0.83
CPX-4273
EmrKY-TolC multidrug efflux transport system
With 6 copies of emrK and 3 copies of emrY
ipTM 0.58* pTM 0.61*
CPX-4443
Sodium/lithium ABC transporter complex
With 1 copy each of ybhS, ybhR, and ybhF
ipTM 0.74 pTM 0.77
CPX-4601
Glutathione/cysteine ABC exporter complex
With 1 copy each of cydC and cydD
ipTM 0.82 pTM 0.76
CPX-4683
N,N′-diacetylchitobiose-specific enzyme II complex
With 2 copies each of chbB and chbC
ipTM 0.46 pTM 0.57
CPX-5888
ygaZH putative valine exporter complex
With 2 copies each of ygaZ and ygaH
ipTM 0.3 pTM 0.39
CPX-5941
Fructose-specific enzyme II complex
With 2 copies of fruA and 4 copies of fruB
ipTM 0.3 pTM 0.36
CPX-5967
L-ascorbate-specific enzyme II complex
With 2 copies of ulaA, and 1 copy each of ulaB and ulaC
ipTM 0.55 pTM 0.66
CPX-6020
MdtIJ spermidine export complex
With 1 copy of mdtI, and 2 copies of mdtJ
ipTM 0.24 pTM 0.35
No inner membrane complexes match.
* ipTM and pTM scores calculated as an average of their two parts (complexes too large to model as one AlphaFold prediction). In all cases, the two parts’ scores were fairly similar (difference < 0.2) and so an average was deemed acceptable.
Outer Membrane Transport Complexes
These complexes include a transporter protein sitting in the outer membrane, and a complex of proteins in the inner membrane. Their inner membrane complexes are all the same: 6 copies of exbB, 3 copies of exbD, and a single tonB which spans the periplasmic space (first card). The remaining cards show the outer membrane transporter proteins.
Inner membrane
ExbBD-TonB complex
6 copies of exbB, 3 copies of exbD and a single tonB
ipTM 0.63 pTM 0.66
No outer membrane complexes match.